Spontaneous Cross-link of Mutated α1 Subunits during GABAA Receptor Assembly
نویسندگان
چکیده
منابع مشابه
Spontaneous Cross - link of Mutated 1 Subunits during GABAA Receptor Assembly
-Aminobutyric acid, type A (GABAA) receptor 1 subunits containing a cysteine mutation at a position in the channel mouth (H109C) surprisingly formed a spontaneous cross-link with each other in receptors composed of 1H109C, 3, and 2 subunits. Cross-linking of two 1H109C subunits didnot significantly change the affinity of [3H]muscimol or [3H]Ro15-1788 binding in 1H109C 3 2 receptors, but GABA di...
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OBJECTIVE To search for antibodies against neuronal cell surface proteins. METHODS Using immunoprecipitation from neuronal cultures and tandem mass spectrometry, we identified antibodies against the α1 subunit of the γ-aminobutyric acid A receptor (GABAAR) in a patient whose immunoglobulin G (IgG) antibodies bound to hippocampal neurons. We searched 2,548 sera for antibodies binding to GABAAR...
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Background γ-Aminobutyric acid (GABA) is considered to be the predominant inhibitory neurotransmitter in mammalian central nervous systems (CNS). There are two major classes of GABA receptors: GABAARs and GABABRs. The GABAA receptor is derived from various subunits such as alpha1-alpha 6, beta1-beta 3, gamma1-gamma 4, delta, epsilon, pi, and rho1-3. Intensive research has been performed to und...
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Objective(s) In this study we investigated the expression of GABAA receptor subunits during brain development. These receptors may change in the embryonic chick forebrain. Materials and Methodes The expression levels of four types of GABAA receptor gamma subunits (γ1, γ2, γ3 and γ4) were quantified in the embryonic chick forebrain at 32 hr, 3, 7, 14, and 20 days of incubation and day one aft...
متن کاملIdentification of amino acid residues important for assembly of GABAA receptor a1 and c2 subunits
Comparative models of GABAA receptors composed of a1b3c2 subunits were generated using the acetylcholinebinding protein (AChBP) as a template and were used for predicting putative engineered cross-link sites between the a1 and the c2 subunit. The respective amino acid residues were substituted by cysteines and disulfide bond formation between subunits was investigated on co-transfection into hu...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2007
ISSN: 0021-9258
DOI: 10.1074/jbc.m609676200